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生技產業創新創業人才培育課程
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黃奇英 教授
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    黃奇英教授
    • 國立陽明大學生物藥學研究所 所長
      • 臨床醫學研究所 教授
      • 生物藥學研究所 合聘
      • 生物醫學資訊研究所 合聘
      • 醫學生物技術暨檢驗學系暨研究所 合聘
    • 辦公室: (112)台北市立農街二段155號圖資大樓623室
    • 電話: 02-28267000 分機7904/6047/6048
    • 傳真: 02-28224045
    • Email: cyhuang5at.pngym.edu.tw;

                                          cyhuang1963at.pngyahoo.com.tw

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• 草藥物的研究開發
近年來以中草藥作為疾病(例如慢性肝臟疾病)替代療法的例子大幅增加,我們以本土植物為開發重點,經過幾年的努力我們已經證實部分純化過的石蓮花對治療肝硬化及肝癌有顯著的效果,目前正與生技中心合作進行製程放大與管控(CMC),預計明年向美國FDA申請植物藥臨床試驗審查(IND)。

 

• 老藥新用藥物篩選平台
這個平台是以基因表現圖譜為基礎,首先針對疾病或藥物以基因微陣列晶片做分析,再透過與生物資訊的結合,利用Connectivity map-簡稱cmap,一個儲存了大量基因表現圖譜的基因微陣列資料庫-將疾病、基因表現及藥物三者做一個連結,快速的找出治療癌症的藥物;使用cmap除了加速藥物篩選外,因為其中包含許多老藥的基因表現圖譜,從老藥切入能夠大幅降低後續臨床試驗的失敗率也是一項關鍵的優勢。

 

  肝纖維化及肝癌藥物
      而在肝癌的部分,我們建立了一個肝癌病人基因表現圖譜的資料庫,進一步透過cmap比對找出可逆轉腫瘤不正常基因表現的潛力藥物,而參與在其中的分子,便可作為將來治療肝癌的標的。除了石蓮花這個植物新藥外我們還找到兩種小分子藥物,且已證實在動物實驗中都可以抑制腫瘤的生長,由此可知,我們所設計的以基因表現圖譜為基礎的比對平台及藥物篩選實驗設計,確實可以發展為一種對抗癌症的治療方式。

   突破抗藥性
      肺癌治療的瓶頸在於藥物具有抗藥性及高復發率的問題,欲突破這個瓶頸我們從癌症幹細胞的基因表現變化來比對cmap中的資料,透過上述的篩選平台我們找到兩個精神疾病用藥可以有效殺死肺癌癌症幹細胞,並能克服抗藥性的問題,且其效果已在動物實驗模式上得到證實,目前已經準備進入臨床試驗。

  跨領域技術平台
      由於傳統基因晶片的單價高,不適合用在大規模篩選,因此我們採用了另一種新的基因表達檢測平台—L1000,速度快且低成本;我們首先利用這個方式系統化的驗證中草藥的效果,且針對其來源品質及成分組成比例做管控,此外也能藉由與小分子藥物的比對推測出中草藥的作用機轉。利用這個優勢不僅能夠更快速且全面性的篩選出具有潛力的藥,過去一年我們已經運用這個新的技術平台得到數十個小分子抗癌藥物及中草藥的基因表現圖譜,未來將透過L1000及cmap的結合加速臨床藥物的開發。

Established Websites
Current Grants
  1. Re-write the pathways in cancer from KEGG via protein-protein interaction network (藉由蛋白質交互作用網路重建KEGG 的訊息傳遞網路) (2010-2013)國科會
  2. From HCC Therapeutic Target Identification to Potential Treatment Strategy (從肝癌治療標的識別到治療策略) (2011-2014)國衛院
  3. 利用電腦模擬藥物篩選平台解析中草藥之表現特徵來發展其生物仿製藥物(2010-2013)經濟部
  4. The identification of potential anti-cancer indications via drug repurposing for lung Adenocarcinoma (從現有臨床藥物中辨識抗肺腺癌的新適應症) (2011-2014)國科會
Professional Experience
  • Professor and chairman in Institute of Biopharmaceutical Sciences, National Yang-Ming University (陽明大學生藥所) (2011-present)
  • Join appointments: Institute of Biopharmaceutical Sciences, Institute of BioMedical Informatics and Institute of Biotechnology in Medicine, National Yang-Ming University (陽明大學生藥所,生資所,醫技所合聘)
  • Associate Investigator in Institute of Cancer Research, National Health Research Institutes (國家衛生研究院癌症研究所) (2005-2007)
  • Associate Investigator in Division of Molecular and Genomic Medicine, NHRI (國家衛生研究院分子與基因醫學組) (2003-2005)
  • Adjunct Associate Professor in Department of Computer Science and Information Engineer, National Taiwan University (台灣大學資訊工程研究所) (2003-2009)
  • Adjunct Associate Professor in Institute of Biotechnology in Medicine and Institute of Bio-Pharmaceutical Sciences, National Yang-Ming University (陽明大學生物醫學技術研究所(2003-2007)及生物藥學研究所(2006-2007)
  • Adjunct Associate Professor in Graduate Institute of Life Sciences, National Defense Medical Center (國防大學生命科學研究所) (1999-2007)
  • Assistant Investigator in Division of Molecular and Genomic Medicine (National Health Research Institutes) (1998-2003)
  • Postdoctoral Fellow (Stanford University) (1994-1998) (Laboratory of James E. Ferrell, Jr.)
  • Ph.D. in Biochemistry and Biophysics (Iowa State University) (1988-1994) (Laboratory of Donald J. Graves)
  • Research Assistant in Institute of Botany (Academia Sinica) (1987-1988) (Laboratory of Li-Chun Huang)
  • Military Service (Taiwan) (1985-1987)
  • B.S. in Chemistry (Tunghai University) (1981-1985)
Honors
  • Leukemia Society of America Career Development Award (1998-1999)
  • Leukemia Society of America Fellowship (1996-1998)
  • Keystone Symposium Travel Award (1997)
  • Stanford Dean’s Postdoctoral Fellowship (1994-1995)
  • Graduate Research Excellence Award (Iowa State University) (1994)
  • The Honor Society of Agriculture (Gamma Sigma Delta) (1989)
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Publications (2004-present)
  1.  Chi-Ying F. Huang, Chiun-Jye Yuan, Nataliya B. Livanova, and Donald J. Graves.  Expression, purification, characterization, and deletion mutations of phosphorylase kinase g subunit: identification of an inhibitory domain in the g subunit. Mol. Cell. Biochem. 127/128, 7-18 (1993).
  2. Chiun-Jye Yuan, Chi-Ying F. Huang, and Donald J. Graves. Phosphorylase kinase: a metal ion-dependent dual specificity kinase. J. Biol. Chem. 268, 17683-17686 (1993).
  3.  Chi-Ying F. Huang, Chiun-Jye Yuan, Siquan Luo, and Donald J. Graves. Mutational analyses of the metal ion binding sites of phosphorylase kinase g subunit: a metal ion-dependent dual specificity kinase. Biochemistry 33, 5877-5883 (1994).
  4.  Chiun-Jye Yuan, Chi-Ying F. Huang, and Donald J. Graves. Oxidation and site-directed mutagenesis of sulfhydryl groups of a truncated g catalytic subunit of phosphorylase kinase. J. Biol. Chem. 269, 24367-24373 (1994).
  5.  Chi-Ying F. Huang, Chiun-Jye Yuan, Nataliya B. Livanova, and Donald J. Graves. Purification and characterization of truncated g1-300 subunit of phosphorylase kinase. Modern Enzymology: Problems and Trends, pp191-205 (1994).
  6.  Siquan Luo, Chi-Ying F. Huang, John F. McClelland, and Donald J. Graves. A study of protein secondary structures by photoacoustic infrard spectroscopy. Anal. Biochem. 216, 67-76 (1994).
  7.  Chi-Ying F. Huang, Chiun-Jye Yuan, Donald K. Blumenthal, and Donald J. Graves. Identification of the substrate and pseudosubstrate binding sites of phosphorylase kinase g subunit. J. Biol. Chem. 270, 7183-7188 (1995).
  8.  Chi-Ying F. Huang and James E. Ferrell, Jr. Ultrasensitivity in the MAP kinase cascade. Proc. Natl. Acad. Sci. USA 93, 10078-10083 (1996).
  9.  Chi-Ying F. Huang and James E. Ferrell, Jr. Dependence of Mos-induced Cdc2 activation on MAP kinase function in a cell-free system. EMBO J. 15, 2169-2173 (1996).
  10.  Chi-Ying F. Huang, Chao-Pei Betty Chang, Chia-Lin Huang, and James E. Ferrell, Jr. M-phase phosphorylation of cytoplasmic dynein intermediate chain and p150Glued. J. Biol. Chem. 274, 14262-14269 (1999).
  11.  See-Chang Huang, Chi-Ying F. Huang, and Te-Chang Lee. Induction of mitosis-mediated apoptosis by sodium arsenite in HeLa S3 cells. Biochem. Pharmacol. 60, 771-780 (2000).
  12.  Wey-Jinq Lin, Yaun-Fu Chang, Wei-Li Wang, and Chi-Ying F. Huang. The mitogen-stimulated TIS21 protein interacts with a protein kinase Ca binding protein rPICK1. Biochemical J. 354, 635-643 (2001).
  13.  Yi-Mi Wu, Chia-Lin Huang, Hsing-Jien Kung, and Chi-Ying F. Huang*. Proteolytic activation of Etk/Bmx tyrosine kinase by caspases. J. Biol. Chem. 276, 17672-17678 (2001).
  14.  Chi-Ying F. Huang, Yi-Mi Wu, Chiung-Yueh Hsu, Wan-Shu Lee, Ming-Derg Lai, Te-Jung Lu, Chia-Lin Huang, Tzeng-Horng Leu, Hsiu-Ming Shih, Hsin-I Fang, Dan R. Robinson, Hsing-Jien Kung, and Chiun-Jye Yuan. Caspase activation of mammalian sterile 20-like kinase 3 (Mst3): nuclear translocation and induction of apoptosis. J. Biol. Chem. 277, 34367-34374 (2002).
  15.  Chiu-Ya Wang, Huey-Jing Lei, Chi-Ying F. Huang, Zhongjian Zhang, Anil B. Mukherjee, and Chiun-Jye Yuan. Induction of cyclooxygenase-2 by staurosporine through the activation of nuclear factor for IL-6 (NF-IL6) and activator protein 2 (AP2) in an osteoblast-like cell line. Biochem. Pharmacol. 64, 177-184 (2002).
  16.  Ann-Ping Tsou#, Chu-Wen Yang#, Chi-Ying F. Huang#, Chang-Tze R. Yu, Yuan-Chii G. Lee, Cha-Wei Chang, Bo-Rue Chen, Yu-Fang Chung, Ming-Ji Fann, Chin-Wen Chi, Jen-Hwey Chiu, and Chen-Kung Chou. Identification of a novel cell cycle regulated gene, HURP, overexpressed in human hepatocellular carcinoma. Oncogene 22, 298-307 (2003) (#contributed equally).
  17.  Jin-Yuan Shih, Yuan-Chii G. Lee, Shuenn-Chen Yang, Tse-Ming Hong, Chi-Ying F. Huang, and Pan-Chyr Yang. Collapsin Response Mediator Protein-1: a novel invasion-suppressor gene. Clinical and Experimental Metastasis 20, 69-74 (2003).
  18.  Chang-Han Chen, Shen-Long Howng, Tai-Shan Cheng, Meng-Hui Chou, Chi-Ying F. Huang, and Yi-Ren Hong. Molecular characterization of human ninein protein: Two distinct subdomains required for centrosomal targeting and regulating signals in cell cycle. Biochem. Biophys. Res. Comm.308, 975-983 (2003).
  19.  Wei-Li Wang, Sheau-Farn Yeh, Yuan-I Chang, Shun-Fang Hsiao, Wei-Nan Lian, Chi-Hung Lin, Chi-Ying F. Huang, Wey-Jinq Lin. PICK1, an Anchoring Protein That Specifically Targets Protein Kinase Ca to Mitochondria Selectively upon Serum Stimulation in NIH 3T3 Cells. J. Biol. Chem. 278, 37705-37712 (2003)
  20. An-Chi Tien, Ming-Hong Lin, Li-Jen Su, Yi-Ren Hong, Tai-Shan Cheng, Yuan-Chii G. Lee, Wey-Jinq Lin, Ivan Still, and Chi-Ying F. Huang*. Identification of the substrates and interaction proteins of Aurora kinases from a protein-protein interaction model. Molecular and Cellular Proteomics 3, 93-104 (2004).
  21.  Fu-Hsiung Chang, Chien-Hsin Lee, Ming-Ta Chen, Chun-Chen Kuo, Yi-Lin Chiang, Chi-Ying F. Huang, and Steve Roffler. Surfection: a new platform for transfected cell arrays. Nucleic Acids Res. 32, e33 (2004).
  22.  Jung-Mao Hsu, Yuan-Chii G. Lee, Chang-Tze R. Yu, and Chi-Ying F. Huang*. Fbx7 functions in the SCF complex regulating Cdk1-cyclin B-phosphorylated HURP proteolysis by proline-rich region. J. Biol. Chem. 279, 32592-32602 (2004).
  23.  Wan-Shu Lee, Chiung-Yueh Hsu, Pei-Ling Wang, Chi-Ying F. Huang, Chia-Hua Chang, and Chiun-Jye Yuan. Identification and characterization of the nuclear import and export signals of the mammalian Ste20-like protein kinase 3. FEBS Letters 13, 41-45 (2004).
  24.  Cheng-Yan Kao, D. Frank Hsu, Han-Yu Chuang, Chi-Ying F. Huang, and Kuang-Chi Chen. To combine or not to combine. International Chinese Statistical Association Bulletin, 37-39 (2004).
  25.  Tao-Wei Huang, An-Chi Tien, Wen-Shien Huang, Yuan-Chii G. Lee, Chin-Lin Peng, Huei-Hun Tseng, Cheng-Yan Kao, and Chi-Ying F. Huang*. POINT: a database for the prediction of protein-protein interactions based on the orthologous interactome. Bioinformatics 20, 3273-3276 (2004).
  26.  Jiunn-Chyi Wu, Tzong-Yueh Chen, Chang-Tze R. Yu, Si-Jie Tsai, Jung-Mao Hsu, Ming-Jer Tang, Chen-Kung Chou, Wey-Jinq Lin, Chiun-Jye Yuan and Chi-Ying F. Huang*. Identification of V23RalA-Ser194 as a critical mediator for Aurora-A-induced cellular motility and transformation by small pool expression screening. J. Biol. Chem. 280, 9013-9022 (2005).
  27.  Chi-Chih Cheng, Shu-Mei Yang, Chi-Ying F. Huang, Jung-Chou Chen, Wei-Mao Chang, and Shih-Lan Hsu. Molecular mechanisms of ginsenoside Rh2-mediated G1 growth arrest and apoptosis in human lung adenocarcinoma A549 cells. Cancer Chemotherapy and Pharmacology 55, 531-40 (2005).
  28.  Te-Jung Lu, Chi-Ying F. Huang, Chiun-Jye Yuan, Yuan-Chii Lee, Tzeng-Horng Leu, Wen-Chang Chang, Te-Ling Lu, Wen-Yih Jeng, Ming-Derg Lai. Zinc ion acts as a cofactor for serine/threonine kinase MST3 and has a distinct role in autophosphorylation of MST3. J. Inorg. Biochem. 99, 1306-1313 (2005).
  29.  Chang-Tze Ricky Yu, Jung-Mao Hsu, Yuan-Chii Gladys Lee, Ann-Ping Tsou, Chen-Kung Chou and Chi-Ying F. Huang*. Phosphorylation and stabilization of HURP by Aurora-A: implication of HURP as a transforming target of Aurora-A. Mol. Cell. Biol. 25, 5789-5800 (2005).
  30.  Kuang-Chi Chen, Tse-Yi Wang, Huei-Hun Tseng, Chi-Ying F. Huang and Cheng-Yan Kao. A stochastic differential equation model for quantifying transcriptional regulatory network in Saccharomyces cerevisiae. Bioinformatics 21, 2883-2890 (2005).
  31.  Kuo-Ting Chang, Chi-Ying F. Huang, Chun-Ming Tsai, Chao-Hua Chiu and Ying-Yung Lok. Role of IL-6 in Neuroendocrine Differentiation and Chemosensitivity of Non-Small Cell Lung Cancer. American Journal of Physiology: Lung Cellular and Molecular Physiology 289, 438-445 (2005).
  32.  Kuo-Ting Chang, Chun-Ming Tsai, Yih-Chy Chiou, Chao-Hua Chiu, King-Song Jeng and Chi-Ying F. Huang*. IL-6 induces neuroendocrine de-differentiation and cell proliferation in non-small cell lung cancer cells. American Journal of Physiology: Lung Cellular and Molecular Physiology 289, 446-453 (2005).
  33.  Yong-Shiang Lin, Li-Jen Su, Chang-Tze Ricky Yu, Fen-Hwa Wong, Hsu-Hua Yeh, Su-Liang Chen, Jiunn-Chyi Wu, Wey-Jinq Lin, Yow-Ling Shiue, Hsiao- Sheng Liu, Shih-Lan Hsu, Jin-Mei Lai and Chi-Ying F. Huang*. Gene Expression Profiles of the Aurora Family Kinases. Gene Expression 13, 15-26 (2006). 
  34.  Shih-Lan Hsu, Chang-Tze Ricky Yu, Sui-Chu Yin, Ming-Jer Tang, An-Chi Tien, Yi-Mi Wu, and Chi-Ying F. Huang*. Caspase 3, periodically expressed and activated at G2/M transition, is required for nocodazole-induced mitotic checkpoint. Apoptosis 11, 765-771 (2006).
  35.  Cheng-Ming Lee, Shih-Yin Chen, Yuan-Chii G. Lee, Chi-Ying F. Huang and Yi-Ming Arthur Chen. Benzo[a]pyrene and Glycine N-methyltransferse Interactions: Gene Expression Profiles of the Liver Detoxification Pathway. Toxicology and Applied Pharmacology 214, 126-135 (2006).
  36.  Li-Jen Su, Shih-Lan Hsu, Jyh-Shyue Yang, Huei-Hun Tseng, Shiu-Feng Huang, and Chi-Ying F. Huang*. Global gene expression profiling of dimethylnitrosamine induced liver fibrosis: from pathological and biochemical data to microarray analysis. Gene Expression 13, 107-132 (2006).
  37.  He-Yen Chou, Shen-Long Howng, Tai-Shan Cheng, Yun-Ling Hsiao, Ann-Shung Lieu, Joon-Khim Loh, Shiuh-Lin Hwang, Ching-Chih Lin, Ching-Mei Hsu, Chihuei Wang, Chu-I Lee, Pei-Jung Lu, Chen-Kung Chou, Chi-Ying F. Huang and Yi-Ren Hong. GSKIP is homologous to the Axin GSK3beta interaction domain and functions as a negative regulator of GSK3beta. Biochemistry 45, 11379-11389 (2006).
  38.  Ya-Shih Tseng, Ching-Cherng Tzeng, Chi-Ying F. Huang, Ping-Hong Chen, Allen Wen-Hsiang Chiu, Pei-Yin Hsu, Guan-Cheng Huang, Yu-Chun Wang, Hsiao-Sheng Liu. Aurora-A overexpression associates with Ha-ras codon-12 mutation and blackfoot disease endemic area in bladder cancer. Cancer Letters 241, 93-101 (2006).
  39.  Te-Jung Lu, Wen-Yang Lai, Chi-Ying F. Huang, Wan-Jung Hsieh, Jau-Song Yu, Ya-Ju Hsieh, Wen-Tsan Chang, Tzeng-Horng Leu, Wen-Chang Chang, Woei-Jer Chuang, Ming-Jer Tang, Tzong-Yueh Chen, Te-Ling Lu, Ming-Derg Lai. Inhibition of cell migration by autophosphorylated mammalian sterile 20-like kinase 3 (MST3) involves paxillin and protein tyrosine phosphatase (PTP)-PEST. J. Biol. Chem. 281, 38405-38417 (2006).
  40.  Yuan-Chii Gladys Lee, Yu-Chyi Hwang, Kuang-Chi Chen, Yong-Shiang Lin, Dah-Yeou Huang, Tao-Wei Huang, Cheng-Yan Kao, Han-Chung Wu, Chin-Tarng Lin, Chi-Ying F. Huang*. Effect of Epstein-Barr Virus Infection on Global Gene Expression in Nasopharyngeal Carcinoma. Functional and Integrative Genomics 7, 79-93 (2007).
  41.  Kuan-Yu Chen, Yuan-Chii Gladys Lee, Jin-Mei Lai, Yih-Leong Chang, Yung-Chie Lee, Chong-Jen Yu, Chi-Ying F. Huang*, Pan-Chyr Yang*. Identification of Trophinin as an Enhancer for Cell Invasion and a Prognostic Factor for Early Stage Lung Cancer. European Journal of Cancer 43, 782-90 (2007). (*corresponding author)
  42.  Tai-Shan Cheng, Yun-Ling Hsiao, Ching-Chih Lin, Ching-Mei Hsu, Mau-Sun Chang, Chu-I Lee, Ricky Chang-Tze Yu, Chi-Ying F. Huang, Shen-Long Howng, and Yi-Ren Hong. hNinein is required for targeting spindle-associated protein Astrin to the centrosome during the S and G2 phases. Exp. Cell. Res. 313, 1710-1721 (2007).
  43.  Chun-Nan Hsu, Jin-Mei Lai, Chia-Hung Liu, Huei-Hun Tseng, Chih-Yun Lin, Kuan-Ting Lin, Hsu-Hua Yeh, Ting-Yi Sung, Wen-Lian Hsu, Li-Jen Su, Sheng-An Lee, Chan-Han Chen, Gen-Cher Lee, Der-Tsai Lee, Yow-Ling Shiue, Chang-Wei Yeh, Chao-Hui Chang, Cheng-Yan Kao, Chi-Ying F. Huang*. Detection of the inferred interaction network in hepatocellular carcinoma from EHCO (Encyclopedia of Hepatocellular Carcinoma genes Online). BMC Bioinformatics 8, 66 (2007).
  44.  Chang-Han Chen, Pei-Jung Lu, Yu-Chia Chen, Shu-Ling Fu, Kou-Juey Wu, Ann-Ping Tsou, Yuan-Chii Gladys Lee, Tsu-Chun Emma Lin, Shih-Lan Hsu, Wey-Jinq Lin, Chi-Ying F. Huang* and Chen-Kung Chou*. FLJ10540-elicited cell transformation is through the activation of PI3-kinase/AKT pathway. Oncogene 26, 4272-4283 (2007) (*corresponding author)
  45.  Li-Jen Su, Ching-Wei Chang, Yu-Chung Wu, Kuang-Chi Chen, Chien-Ju Lin, Shu-Ching Liang, Chi-Hung Lin, Jacqueline Whang-Peng, Shih-Lan Hsu, Chen-Hsin Chen and Chi-Ying F. Huang*. Selection of DDX5 as a Novel Internal Control for Q-RT-PCR from microarray data: Using a Block Bootstrap Re-sampling scheme. BMC Genomics 8(1),140 (2007).
  46.  Chueh-Chuan Yen, Shu-Ching Liang, Yiin-Jeng Jong, Yann-Jang Chen, Chi-Hung Lin, Yuh-Min Chen, Yu-Chung Wu, Wu-Chou Su, Chi-Ying F. Huang, Szu-Wen Tseng and Jacqueline Whang-Peng. Chromosomal aberrations of malignant pleural effusions of lung adenocarcinoma: Different cytogenetic changes are correlated with genders and smoking habits. Lung Cancer Sep;57(3):292-301 (2007).
  47.  Wei-Li Wang, Sheau-Farn Yeh, Eagle Yi-Kung Huang, Yu-Ling Lu, Chun-Fa Wang, Chi-Ying F. Huang and Wey-Jinq Lin. Mitochondrial anchoring of PKCa by PICK1 confers resistance to etoposide-induced apoptosis. Apoptosis Oct. 12(10):1857-71 (2007).
  48.  Chang-Tze Ricky Yu, Jiunn-Chyi Wu, Mei-Chih Liao, Shih-Lan Hsu and Chi-Ying F. Huang*. Identification of c-Fos as a mitotic phosphoprotein: regulation of c-Fos by Aurora-A. J. Biomedical Sci. 15:79-87 (2008).
  49.  Pao-Ling Torng, Yuan-Chii G. Lee, Chi-Ying F. Huang, Y. H. Ye, Yong-Shiang Lin, Yi-Wen Chu, Su-Cheng Huang, Pinchas Cohen, Cheng-Wen Wu, Chin-Tarng Lin. Insulin-like grow factor binding protein-3 (IGFBP-3) acts as an invasion-metastasis suppressor in ovarian endometrioid carcinoma. Oncogene 3:2137-2147 (2008).
  50.  Tai-Shan Cheng, Yun-Ling Hsiao, Ching-Chih Lin, Ricky Chang-Tze Yu, Ching-Mei Hsu, Mau-Sun Chang, Chu-I Lee, Chi-Ying F. Huang, Shen-Long Howng, and Yi-Ren Hong. Glycogen synthase kinase 3beta interacts with and phosphorylates the spindle-associated protein astrin. J. Biol. Chem 283(4):2454-2464 (2008).
  51.  Dah-Yeou Huang, Yi-Ting Lin, Pey-Shynan Jan, Yu-Chyi Hwang, Sung-Tzu Liang, Yeh Peng, Chi-Ying F. Huang, Han-Chung Wu and Chin-Tang Lin. Transcription factor SOX-5 enhances nasopharyngeal carcinoma progression by downregulating SPARC gene expression. J. Pathology 214(4): 445-455 (2008).
  52.  Chia-Ying Yang, Chao-Hui Chang, Tsu-Chun Emma Lin, Ya-Ling Yu, Sheng-An Lee, Chueh-Chuan Yen, Jinn-Moon Yang, Jin-Mei Lai, Yi-Ren Hong, Tzu-Ling Tseng, Kun-Mao Chao and Chi-Ying F. Huang*. PhosphoPOINT: a comprehensive human kinase interactome and phospho-protein database. Bioinformatics 24, i14-i20 (2008).
  53.  Sheng-An Lee, Cheng-hsiung Chan, Chi-Hung Tsai, Jin-Mei Lai, Feng-Sheng Wang, Cheng-Yan Kao and Chi-Ying F. Huang*. Ortholog-based protein-protein interaction prediction and its application to inter-species interactions. BMC Bioinformatics 9(Suppl 12):S11 (2008).
  54.  Fen-Hwa Wong#, Chi-Ying F. Huang#, Li-Jen Su, Yu-Chung Wu, Yong-Shiang Lin, Jiun-Yi Hsia, Hsin-Ting Tsai, Sheng-An Lee, Chi-Hung Lin, Cheng-Hwai Tzeng, Po-Min Chen, Yann-Jan Chen, Shu-Ching Liang, Jin-Mei Lai, and Chueh-Chuan Yen. Combination of microarray profiling and protein-protein interaction databases delineates the minimal discriminators as a metastasis network for esophageal squamous cell carcinoma. Int J Oncology 34: 117-128 (2009). (#contributed equally)
  55.  Zhong-Zhe Lin, Hey-Chi Hsu, Chih-Hung Hsu, Pei-Yen Yeh, Chi-Ying F. Huang, Yung-Feng Huang, Te-Jung Chen, Sung-Hsin Kuo, Chiun Hsu, Fu-Chang Hu, Yung-Ming Jeng, Ying Chung, and Ann-Lii Cheng. The Aurora kinase inhibitor VE-465 has anticancer effects in pre-clinical studies of human hepatocellular carcinoma. J. Hepatology 50(3): 518-527 (2009).
  56.  Chang-Han Chen, Jin-Mei Lai, Teh-Ying Chou, Cheng-Yu Chen, Li-Jen Su, Yuan-Chii Lee, Tai-Shan Cheng, Yi-Ren Hong, Jacqueline Whang-Peng, Chen-Kung Chou, Yu-Chung Wu, and Chi-Ying F. Huang*. VEGFA upregulates FLJ10540 and modulates migration and invasion of lung cancer via PI3K/AKT pathway. PLoS ONE 4(4): e5052 (2009).
  57.  Sheng-An Lee, Chen-Hsiung Chan, Tzu-Chi Chen, Chia-Ying Yang, Kuo-Chuan Huang, Chi-Hung Tsai, Jin-Mei Lai, Feng-Sheng Wang, Cheng-Yan Kao, Chi-Ying F. Huang*. POINeT: Protein Interactome with Sub-network Analysis and Hub Prioritization. BMC Bioinformatics 10:114 (2009).
  58.  Chang-Han Chen, Chih-Yen Chien, Chao-Cheng Huang, Hui-Ching Chuang, Fu-Min Fang, Hsuan-Ying Huang, Ching-Mei Chen, Hsin-Ling Liu and Chi-Ying F. Huang*. Expression of FLJ10540 is correlated with aggressiveness of oral cavity squamous cell carcinoma by stimulating cell migration and invasion via increased FOXM1 and MMP-2 activity. Oncogene 28, 2723-2737 (2009). (IF = 6.37)
  59.  Tzu-Chi Chen, Sheng-An Lee, Chen-Hsiung Chan, Yue-Li Juang, Yi-Ren Hong, Yi-Shann Huang, Jin-Mei Lai, Cheng-Yan Kao, and Chi-Ying F. Huang*. Cliques in mitotic spindle network bring kinetochore-associated complexes to form dependence pathway. Proteomics 9, 4048-4062 (2009) (with a figure selected as journal cover.)
  60.  Shun-Fu Chen, Yue-Li Juang, Wei-Kang Chou, Jin-Mei Lai, Chi-Ying F Huang*, Cheng-Yan Kao, and Feng-Sheng Wang*. Inferring a transcriptional regulatory network of the cytokinesis-related genes by network component analysis. BMC Systems Biology 3:110 (2009). (*corresponding author)
  61.  Tzu-Chi Chen, Sheng-An Lee, Tse-Ming Hong, Jin-Yuan Shih, Jin-Mei Lai, Hsin-Ying Chiou, Shuenn-Chen Yang, Chen-Hsiung Chan, Cheng-Yan Kao, Pan-Chyr Yang* and Chi-Ying F. Huang*. From midbody protein-protein interaction network construction to novel regulators in cytokinesis. Journal of Proteome Research 8, 4943-4953 (2009). (IF = 5.11)
  62.  Ya-Shih Tseng, Jheng-Chang Lee, Chi-Ying F. Huang and Hsiao-Sheng Liu. Aurora-A overexpression enhances cell-aggregation of Ha-ras transformants through the MEK/ERK signaling pathway. BMC Cancer 9:435 (2009).
  63.  Ferrell JE, Pomerening JR, Kim SY, Trunnell NB, Xiong W, Huang CY, Machleder EM. Simple, realistic models of complex biological processes: positive feedback and bistability in a cell fate switch and a cell cycle oscillator. FEBS Lett. 583 (24): 3999-4005 (2009)
  64.  Huang CW, Lin CY, Huang HY, Liu HW, Chen YJ, Shih DF, Chen HY, Juan CC, Ker CG, Huang CY, Li CF, Shiue YL. CKS1B overexpression implicates clinical aggressiveness of hepatocellular carcinomas but not p27(Kip1) protein turnover: an independent prognosticator with potential p27 (Kip1)-independent oncogenic attributes? Ann Surg Oncol. 17(3):907-22 (2010).
  65.  Ann-Shung Lieu, Tai-Shan Cheng, Chia-Hua Chou, Chia-Hung Wu, Chia-Yi Hsu, Chi-Ying F. Huang, Li-Kwan Chang, Joon-Khim Loh, Chung-Shing Chang,Ching-Mei Hsu, Shen-Long Howng And Yi-Ren Hong. Functional characterization of AIBp, a novel Aurora-A binding protein in centrosome structure and spindle formation. International Journal Of Oncology (37): 429-436 (2010)
  66.  Yu-Chung Wu, Li-Jen Su, Hao-Wei Wang, Chien-Fu Jeff Lin, Wen-Hu Hsu, Teh-Ying Chou, Chi-Ying F. Huang, Chia-Li Lu, and Chung-Tsen Hsueh. Cooverexpression of Cyclooxygenase-2 and Microsomal Prostaglandin E Synthase-1 Adversely Affects the Postoperative Survival in Non-small Cell Lung Cancer. Journal of Thoracic Oncology 5(8):1167-74 (2010).
  67.  Yun-Chih Hsieh, Yerra Koteswara Rao, Chun-Chi Wu, Chi-Ying F. Huang, Madamanchi Geethangili, Shih-Lan Hsu, and Yew-Min Tzeng. Methyl Antcinate A from Antrodia camphorata Induces Apoptosis in Human Liver Cancer Cells through Oxidant-Mediated Cofilin- and Bax-Triggered Mitochondrial Pathway. Chem Res Toxicol. 23(7):1256-67 (2010).
  68.  Yin-Ju Chen, Yu-Hsuan Chen, Lu-Ping Chow, Ya-Hui Tsai, Pei-Hong Chen, Chi-Ying F. Huang, Wei-Tzu Chen, and Lih-Hwa Hwang. Heat shock protein 72 is associated with the hepatitis C virus replicase complex and enhances viral RNA replication. J Biol Chem 285, 28183-28190 (2010).
  69.  Ming-Ying Lan, Chi-Long Chen, Kuan-Ting Lin, Sheng-An Lee, Wu-Lung R. Yang, Chun-Nan Hsu, Jaw-Ching Wu, Ching-Yin Ho, Jin-Ching Lin, Chi-Ying F. Huang*. From NPC Therapeutic Target Identification to Potential Treatment Strategy. Molecular Cancer Therapeutics 9(9):2511-23 (2010). (IF = 5.22)
  70.  Chia-Hung Liu#, Kuan-Ting Lin#, Chi-Ying F. Huang#, Yih-Jyh Shann, Yu-Shi Lin, Cheng-Yan Kao, Chun-Nan Hsu. Genome-Wide Detection of Putative Oncofetal Genes in Human Hepatocellular Carcinoma by Splicing Pattern Comparison. iConcept Transaction on Computational Intelligence in Bioinformatics, 2:1, iConcept Press (2010) (#contributed equally)
  71.  Sheng-Da Hsu, Feng-Mao Lin, Wei-Yun Wu, Chao Liang, Wei-Chih Huang, Wen-Ling Chan, Wen-Ting Tsai, Goun-Zhou Chen, Chia-Jung Lee, Chih-Min Chiu, Chia-Hung Chien, Ming-Chia Wu, Chi-Ying Huang, Ann-Ping Tsou and Hsien-Da Huang. miRTarBase: a database curates experimentally validated microRNA-target interactions. Nucleic Acids Research 39:D163–D169 (2011).
  72.  Yuan-I Chang, Sheng-Chieh Hsu, Gar-Yang Chau, Chi-Ying F. Huang, Jung-Sung Sung, Wei-Kai Hua, Wey-Jinq Lin. Identification of the methylation preference region in heterogeneous nuclear ribonucleoprotein K by protein arginine methyltransferase 1 and its implication in regulating nuclear/cytoplasmic distribution. Biochemical and Biophysical Research Communications 404(3):865–869 (2011).
  73.  Hsien-Chia Juan, Hsin-Ting Tsai, Po-Hao Chang, Chi-Ying F. Huang, Cheng-Po Hu and Fen-Hwa Wong. Insulin-like growth factor 1 mediates 5-fluorouracil chemoresistance in esophageal carcinoma cells through increasing survivin stability. Apoptosis 16(2):174-183 (2011).
  74.  Chien-Chung Lin, Lien-Chin Chen, Vincent S. Tseng, Jing-Jou Yan, Wu-Wei Lai, Wen-Pin Su, Chi-Hung Lin, Chi-Ying F. Huang, and Wu-Chou Su. Malignant pleural effusion cells show aberrant glucose metabolism gene expression. European Respiratory Journal 37(6): 1453–1465 (2011).
  75.  Yu-Ying Lin, Chun-Hung Yang, Gwo-Tarng Sheu, Chi-Ying F. Huang, Yu-Chung Wu, Shu-Ming Chuang, Ming-Ji Fann, Han Chang, Huei Lee, Jinghua Tsai Chang. A novel exon 15-deleted, splicing variant of slit2 shows potential for growth inhibition in addition to invasion inhibition in lung cancer. Cancer 117(15): 3404-15 (2011).
  76.  Jiz-Yuh Wang, Chi-Ling Yeh, Hsiao-Chin Chou, Chi-Hwa Yang, Yu-Ning Fu, Ya-Ting Chen, Hui-Wen Cheng, Chi-Ying F. Huang, Hui-Ping Liu, Shiu-Feng Huang and Yi-Rong Chen. Vaccinia H1-related phosphatase (VHR) is a phosphatase of ErbB receptors and is down-regulated in non-small cell lung cancer. The Journal of Biological Chemistry 286(12): 10177–10184 (2011).
  77.  Yei-Hsuan Huang, Chun-Chi Wu, Chen-Kung Chou, and Chi-Ying F. Huang*. A Translational Regulator, PUM2, Promotes both Protein Stability and Kinase Activity of Aurora-A. PLoS ONE 6(5): e19718 (2011)
  78.  Kai-Wei Hsueh, Shu-Ling Fu, Chi-Ying F. Huang, Chao-Hsiung Lin. Aurora-A phosphorylates hnRNPK and disrupts its interaction with p53. FEBS Letters 585, 2671–2675 (2011).
  79.  Hsiao-Sheng Liu, Ching-Shiun Ke, Hung-Chi Cheng, Chi-Ying F. Huang, and Chun-Li Su. Curcumin-induced Mitotic Spindle Defect and Cell Cycle Arrest in Human Bladder Cancer Cells is partly through Inhibition of Aurora-A. Mol. Pharmacol. 80: 638-646 (2011).
  80.  Yun-Chih Hsieh, Yerra Koteswara Rao, Jacqueline Whang-Peng, Chi-Ying F. Huang, Song-Kun Shyue, Shih-Lan Hsu, and Yew-Min Tzeng. Antcin B and Its Ester Derivative from Antrodia camphorata Induce Apoptosis in Hepatocellular Carcinoma Cells Involves Enhancing Oxidative Stress Coincident with Activation of Intrinsic and Extrinsic Apoptotic Pathway. J. Agric. Food Chem. 59: 10943-10954 (2011).
  81.  Ming-Huang Chen, Wu-Lung R. Yang, Kuan-Ting Lin, Chia-Hung Liu,  Yu-Wen Liu, Kai-Wen Huang, Peter Mu-Hsin Chang, Jin-Mei Lai, Chun-Nan Hsu, Kun-Mao Chao, Cheng-Yan Kao, Chi-Ying F. Huang*. Gene Expression-Based Chemical Genomics Identifies Potential Therapeutic Drugs in Hepatocellular Carcinoma. PLoS ONE 6(11): e27186 (2011). 
  82.  Yu-Liang Lee, Jie-Wei Weng, Wen-Chin Chiang, Yi-Wen Lin, Ka-Lok Ng*, Jeffrey Jing-Pha Tsai, Chi-Ying F. Huang*. Investigating Cancer-related Proteins Specific Domain Interactions and Differential Protein Interactions caused by Alternative Splicing, IEEE International Conference on Bioinformatics and Bioengineering BIBE 2011, 1(1), 33-38 (2011) (EI) 
  83.  Yu-Yu Lin, Yung-Ho Hsu, Hsin-Yi Huang, Yih-Jyh Shann, Chi-Ying F. Huang, Shu-Chen Wei, Chi-Ling Chen, and Tzuu-Shuh Jou. Aberrant nuclear localization of EBP50 promotes colorectal carcinogenesis in xenotransplanted mice by modulating TCF-1 and β-catenin interactions. J. Clinical Investigation 122(5): 1881-1894 (2012).
  84.  Li-Jen Su, Chih-Hsueh Yang, Shiu-Feng Huang, Ya-Ling Yuo, Hui-Chu Hsieh, Tzu-Ling Tseng, Chang-Han Chen, Shih-Lan Hsu* and Chi-Ying F. Huang*. Evaluation of the Chinese medicinal herb, Graptopetalum paraguayense, as a therapeutic treatment for liver damage in rat models. Evidence-Based Complementary and Alternative Medicine 2012  (*contributed equally) (2012)
  85.  Chang-Han Chen, Li-Yen Shiu, Li-Jen Su, Chi-Ying F. Huang, Shun-Chen Huang, Chao-Cheng Huang, Yu-Fang Yin, Wei-Sheng Wang, Hsin-Ting Tsai, Fu-Min Fang, Wan-Chu Chuang, Hong-Chang Kang and Chung-Feng Hwang. FLJ10540 is associated with tumor progression in nasopharyngeal carcinomas and contributes to nasopharyngeal cell proliferation and metastasis via the osteopontin/CD44 pathway. Journal of Translational Medicine 10(1): 93 (2012). 
  86.  Yi-Hua Jan, Hong-Yuan Tsai, Chih-Jen Yang, Ming- Shyan Huang, Yi-Fang Yang, Tsung-Ching Lai, Chien-Hsin Lee, Yung-Ming Jeng, Chi-Ying F. Huang, Jen-Liang Su, Yung-Jen Chuang, and Michael Hsiao. Adenylate kinase-4 is a marker of poor clinical outcomes that promotes metastasis of lung cancer by downregulating the transcription factor ATF3. Cancer Research 72(19):5119-29 (2012)
  87.  Chi-Tai Yeh, Alexander TH Wu, Peter M-H Chang, Kuan-Yu Chen, Chia-Ning Yang, Shuenn-Chen Yang, Chao-Chi Ho, Chun-Chi Chen, Yu-Lun Kuo, Pei-Ying Lee, Yu-Wen Liu, Chueh-Chuan Yen, Michael Hsiao, Pei-Jung Lu, Jin-Mei Lai, Liang-Shun Wang, Chih-Hsiung Wu, Jeng-Fong Chiou, Pan-Chyr Yang, and Chi-Ying F. Huang*. Trifluoperazine, an antipsychotic agent, inhibits cancer stem cell growth and overcomes drug resistance of lung cancer. American Journal of Respiratory and Critical Care Medicine 186(11):1180-8 (2012) (IF: 11.080) 
  88.  Wu-Lung R. Yang, Yu-En Lee, Ming-Huang Chen, Kun-Mao Chao*, Chi-Ying F. Huang*. In-silico drug screening and potential target identification for hepatocellular carcinoma using Support Vector Machines based on drug screening result. Gene 518:201-8 (2013) (*contributed equally)
  89.  Ming-Huang Chen, Yi-Chen Yeh, Yee Chao, Chung-Pin Li, Yi-Ming Shyr, Shin-e Wang, Cheng-Hwai Tzeng, Peter Mu-Hsin Chang, Chun-Yu Liu, Ming-Han Chen, Michael Hsiao and Chi-Ying F. Huang*. Expression of Gremlin 1 (GREM1) Correlated With Increased Angiogenesis and Increased Progression-Free Survival in Patients with Pancreatic Neuroendocrine Tumors. Journal of Gastroenterology 48(1):101-8 (2013)
  90.  Ming-Huang Chen, Kun-Ju Lin, Wu-Lung R. Yang, Ya-Wen Kao, Tsung-Wen Chen, Shu-Chaou Chao, Peter Mu-Hsin Chang, Chun-Yu Liu, Cheng-Hwai Tzeng, Yee Chao, Ming-Han Chen, Chun-Nan Yeh, and Chi-Ying F. Huang*. Gene Expression-Based Chemical Genomics Identifies Heat Shock Protein 90 Inhibitors as Potential Therapeutic Drugs in Cholangiocarcinoma. Cancer 119(2):293-303 (2013) (IF:4.771)
  91.  Li-Jen Su, Chia-Chuan Chang, Chih-Hsueh Yang, Shur-Jong Hsieh, Yi-Chin Wu, Jin-Mei Lai, Tzu-Ling Tseng, Chi-Ying F. Huang*, and Shih-Lan Hsu*. Graptopetalum paraguayense ameliorates chemical-induced rat hepatic fibrosis in vivo and inactivates stellate cells and Kupffer cells in vitro. PLoS ONE 8(1): e53988 (2013)
  92.  Yu-Cheng Lee, Po-Chi Liao, Yih-Cherng Liou, Michael Hsiao, Chi-Ying Huang*, and Pei-Jung Lu*. Glycogen synthase kinase 3 β activity is required for hBora/Aurora A-mediated mitotic entry. Cell Cycle 12(6):1-8 (2013) (*contributed equally) (IF: 5.359)
  93.  Pei-Ying Lee, Chi-Long Chen, Zhong-Zhe Lin, Ann-Lii Cheng, Edmund I-Tsuen Chen, Jacqueline Whang-Peng and Chi-Ying F. Huang*. The Aurora kinases inhibitor VE-465 is a novel treatment for glioblastoma multiforme. Oncology 84(6):326-35 (2013) 
  94.  Tzu-Chi Chen, Yu-Wen Liu, Yei-Hsuan Huang, Yi-Chen Yeh, Teh-Ying Chou, Yu-Chung Wu, Chun-Chi Wu, Yi-Rong Chen, Hui-Chuan Cheng, Pei-Jung Lu, Jin-Mei Lai, Chi-Ying F. Huang*. Protein phosphorylation profiling via in situ proximity ligation assay: implication of AURKA-elicited EGFR-Thr654 and EGFR-Ser1046 phosphorylation in EGFR mutant cells. PLoS ONE 8(3):e55657 (2013) 
  95.  Chia-Hung Liu, Tzu-Chi Chen, Gar-Yang Chau, Yi-Hua Jan, Chun-Houh Chen, Chun-Nan Hsu, Kuan-Ting Lin, Yue-Li Juang, Pei-Jung Lu, Hui-Chuan Cheng, Ming-Huang Chen, Chia-Fen Chang, Yu-Shan Ting, Cheng-Yan Kao, Michael Hsiao, and Chi-Ying F. Huang*. An analysis of protein-protein interactions in cross-talk pathwaysreveals CRKL as a novel prognostic marker in hepatocellular carcinoma. Molecular and Cellular Proteomics 12(5):1335-49 (2013) (IF: 7.398
  96.  Jiun-Ming Wu, Chiung-Tong Chen, Mohane Selvaraj Coumar, Wen-Hsin Lin, Zi-Jie Chen, John T.-A. Hsu, Yi-Hui Peng, Hui-Yi Shiao, Wen-Hsing Lin, Chang-Ying Chu, Jian-Sung Wu, Chih-Tsung Lin, Ching-Ping Chen, Ching-Cheng Hsueh, Kai-Yen Chang, Li-Pin Kao, Chi-Ying F. Huang, Yu-Sheng Chao, Su-Ying Wu, Hsing-Pang Hsieh, and Ya-Hui Chi. Aurora kinase inhibitors reveal mechanisms of HURP in nucleation of centrosomal and kinetochore microtubules. Proc Natl Acad Sci U S A. 110(19):E1779-87 (2013) (Published online)
  97.  Yu-Feng Hu, Tzu-Chi Chen, Gar-Yang Chau, Tsung-Lin Yang, Chia-Jen Liu, Ming-Huang Chen, Peter Mu-hsin Chang, Tzeng-Ji Chen, Michael Hsiao, Chi-Ying F. Huang* and Shih-Ann Chen*. Baseline hypertension: New insight into the potential predictors of survival in patients with hepatocellular carcinoma. International Journal of Cardiology (2013) 168(3):2979–2981. (*contributed equally) (IF: 7.078)
  98.  Chien-Hung Huang, Min-You Wu, Chi-Ying F. Huang, and Ka-Lok Ng. Therapeutic target identification for lung cancer, international conference on bioinformatics (ICB'13) (2013) (EI)
  99.  Mu-Hsin Peter Chang, Yi-Chen Yeh, Tzu-Chi Chen, Yu-Chung Wu, Pei-Jung Lu, Hui-Chuan Cheng, Hsueh-Ju Lu, Ming-Huang Chen, Teh-Ying Chou, Chi-Ying F. Huang*. High expression of CHRNA1 is associated with reduced survival in early stage lung adenocarcinoma after complete resection. Annals of Surgical Oncology 20:3648-3654 (2013)
  100.  Chi-Tai Yeh#, Chun-Li Su#, Chi-Ying F. Huang#, Justin Kung-Yi Lin, Wei-Hwa Lee, PeterM.-H. Chang, Yu-Lun Kuo, Yu-Wen Liu, Liang-Shun Wang, Chih-Hsiung Wu, Yi-Shing Shieh, Yi-Hua Jan, Yung-Jen Chuang, Michael Hsiao, and Alexander T. H. Wu. A Preclinical Evaluation of Antimycin A as a Potential Antilung Cancer Stem Cell Agent. Evidence-Based Complementary and Alternative Medicine. 2013; 2013.
  101.  Chih-Cheng Chen, Chia-Bao Chu, Ko-Jiunn Liu, Chi-Ying F. Huang, Jang-Yang Chang, Wen-Yu Pan, Huang-Hui Chen, Yun-Hsia Cheng, Kuan-Der Lee, Miao-Fen Chen, Ching-Chuan Kuo, Li-Tzong Chen. Gene expression profiling for analysis acquired oxaliplatin resistant factors in human gastric carcinoma TSGH-S3 cells: The role of IL-6signaling and Nrf2/AKR1C axis identification. Biochemical Pharmacology 86(7):872–887 (2013).
  102.  Yu-Cheng Lee, Jenny Que, Yu-Chia Chen, Jen-Tai Lin, Yih-Cherng Liou, Po-Chi Liao, Yu-Peng Liu, Kuen-Haur Lee, Li-Ching Lin, Michael Hsiao, Liang-Yi Hung, Chi-Ying Huang* and Pei-Jung Lu*. Pin1 acts as a negative regulator of the G2/M transition through an interplay with the Aurora A/hBora complex. J Cell Sci. 2013 Aug 22. [Epub ahead of print]
  103.  Ming-Ying Lan, Wu-Lung R. Yang, Kuan-Ting Lin, Jin-Ching Lin, Yih-Jyh Shann,  Ching-Yin Ho, Chi-Ying F. Huang*. Using computational strategies to predict potential drugs for nasopharyngeal Carcinoma. HEAD & NECK (in press)
  104.  Kuan-Ting Lin, Yih-JyhShann, Gar-Yang Chau, Chun-Nan Hsu, and Chi-Ying F. Huang*.Identification of latent biomarkers in hepatocellular carcinoma by ultra-deep whole-transcriptome sequencing. Oncogene [Epub ahead of print]
  105.  Tzu-Chi Chen, Sheng-An Lee, Kuan-Ting Lin, Chun-Houh Chen, Hsuan-Cheng Huang, Pei-Ying Lee, Yu-Wen Liu, Yu-Lun Kuo, Ming-Hui Yu, Wilber Huang, Jin-Mei Lai, and Chi-Ying F. Huang*. Using an in situ proximity ligation assay to systematically profile endogenous protein-protein interactions in a pathway network (revised)
  106.  Pei-Jung Wu, Wu-Hsiung Wu, Tzu-Chi Chen, Jin-Mei Lai, Chi-Ying F. Huang, and Feng-Sheng Wang. Reconstruction and analysis of signal transduction network using HeLa cell protein-protein interaction data (revised)

 

Book Chapter

 

 

 

1.       Jin-Mei Lai, Chi-Ying F. Huang, and Chang-Han Chen. Using siRNA to uncover novel oncogenic signaling pathways. RNA interference, Methods in Molecular Biology 623, 231-242 (2010).

 

 

 

Patents
  1. 謝惠珠(Hui-Chu Hsieh), 曾錙翎 (Tzu-Ling Tseng), 蘇立仁 (Li-Jen Su), 黃奇英 (Chi-Ying Huang), 徐士蘭 (Shih-Lan Hsu), “肝纖維化及/或肝硬化之生物標誌及其檢驗方法” (Biomarkers for liver fibrotic injury) (日本發明第4297942號專利權)。(Date of Grant: 24 April, 2009)
  2. 謝惠珠(Hui-Chu Hsieh), 曾錙翎 (Tzu-Ling Tseng), 蘇立仁 (Li-Jen Su), 黃奇英 (Chi-Ying Huang), 徐士蘭 (Shih-Lan Hsu), “Biomarkers for liver fibrotic injury” (新加坡發明第134283號專利權)。(Date of Grant: 30 Nov., 2009)
  3. 謝惠珠(Hui-Chu Hsieh), 曾錙翎 (Tzu-Ling Tseng), 蘇立仁 (Li-Jen Su), 黃奇英 (Chi-Ying Huang), 徐士蘭 (Shih-Lan Hsu), “Biomarkers for liver fibrotic injury” (美國發明第7,972,785號專利權)(Date of Grant: 5 July, 2011)

 

Patents under review

 

 

 

     1.     Anti-Cancer Extract and Components (US, PCT, Taiwan)
     2.     Method for Inhibiting the Growth of Cancer Stem Cells (US, Taiwan)
     3.     Pharmaceutical Composition for Elimination of Cancer Stem Cells (US, PCT)
     4.     Method for selecting a Pool of Molecules (US, PCT)
     5.     Method for Treating Brain Tumor (US)

 

其他協助產業技術發展之具體績效

許多蛋白激酶(protein kinase)為癌症藥物標靶,蛋白激酶磷酸化狀態的改變可反應出細胞內訊息路徑的活化與否,為了分析癌化過程蛋白激酶磷酸化的程度,我們與亞諾法公司合作生產蛋白激酶及磷酸化的蛋白質抗體,為了增加磷酸化抗體的靈敏度與專一性,我們利用PLA分析的方式(即利用蛋白激酶及其磷酸化的配對抗體來偵測細胞內蛋白激酶磷酸化及蛋白質交互作用的情形),以偵測蛋白激酶參與的訊息網絡或細胞癌化的過程,我們的成果如下:
   1. 製造並上市268個以PLA形式配對的磷酸化蛋白質的抗體。
   2. 製造並上市612 個以PLA形式配對的蛋白質交互作用抗體。
   3. 自2009/10/1至2011/12/31,配對的蛋白質磷酸化及蛋白質交互作用抗體(Antibody Pairs for PLA™)之總營收金額為64,888美

       元,亞諾法回饋3%給陽明大學(1,375 美元) 。